Genotoxic effect of Physalis angulata L. (Solanaceae) extract on human lymphocytes treated in vitro

Physalis angulata L (Solanaceae) is a medicinal plant from North of Brazil, whose different extracts and infusions are commonly used in the popular medicine for the treatment of malaria, asthma, hepatitis, dermatitis and rheumatism. However, the genotoxic effects of P. angulata on human cells is not well known. The main purpose of the present study was to evaluate the in vitro genotoxic effects of aqueous extract of P. angulata using the comet assay and the micronucleus assay in human lymphocytes provided from 6 healthy donors. Treatments with P. angulata extracts were performed in vitro in order to access the extent of DNA damage. The comet assay has shown that treatments with P. angulata at 0.5, 1.0, 2.0, 3.0 and 6.0 microg/mL in culture medium were genotoxic. Lymphocytes treated with P. angulata at the concentrations of 3.0 and 6.0 microg/mL in culture medium showed a statistically significant increase in the frequency of micronucleus (p<0.05), however, the cytokinesis blocked proliferation index (CBPI) was not decreased after P. angulata treatment. In conclusion, the present work demonstrated the genotoxic effects of P. angulata extract on human lymphocytes in vitro.     

Large scale transmission network planning using optimization andheuristic techniques

Pursuing optimal solutions for large scale transmission network planning problems is a formidable task due to their combinatorial nature and also due to the nonconvexities involved. Successful approaches using hierarchical Benders decomposition incur in a high computational cost mainly due to the need to solve a large integer program (the investment sub-problem) for every Benders iteration. In this work the authors propose to use heuristics within the decomposition framework, therefore avoiding to solve to optimality each integer sub-problem. The global computational effort is substantially reduced, and allows coping with large problems that would be intractable using classical combinatorial techniques. Case studies with the 6 bus Garver test system and a reduced Southeastern Brazilian power network are presented and discussed     

Sexual disorders: demographic and diagnostic profile during one year of a multidisciplinary project

Taking into consideration a study published 10 years ago on sexual disturbances of students at the University of S?o Paulo, the importance of the subject is discussed, along with the creation in 1993 of the Sexuality Project at the university hospital of the University of S?o Paulo School of Medicine. In its first year, this multidisciplinary project attended 140 patients with sexual dysfunctions (associated or not to other clinical manifestations); the majority were younger than 60 years-old, and 80 percent were male.     

Drying of Chestnuts (Castanea sativa Mill.) after Osmotic Dehydration with Sucrose and Glucose Solutions

Chestnuts were dehydrated by using a combined method of osmotic dehydration followed by air drying. Samples were osmotically pretreated with sucrose (60% w/w) and glucose (56% w/w) for 8 h, air-dried at temperatures of 45, 55, and 65°C, at a relative humidity of 30% and at a velocity of 2.7 m·s^-1 and the experimental data of the drying kinetics were obtained. Whole samples were dried with different peelings: (a) removal of endocarp and pericarp (peeled) and (b) additionally the internal rough surface (cut). In all cases, cut chestnuts show greater drying rates than peeled samples, indicating that a significant mass transfer resistance in the layer nearest to the surface takes place. Peeled samples pretreated with sucrose solutions behave in a similar way to untreated samples. For the rest of the samples, the cut samples osmotically treated with sucrose solutions and all the samples treated with the glucose solution, the drying rates decrease during drying. Drying kinetics are successfully modeled by employing a diffusional model that takes the shrinkage into account. The effective coefficient of water diffusion was evaluated and correlated with temperature. The quality of the final product was monitored by color change. In spite of the fact that the total color difference is not modified by the osmotic treatment, the L*, a*, and b* color coordinates of cut samples treated with sucrose and glucose solutions do undergo changes; the L* and a* coordinates change less than the b*.     

Gas Permeability of Wetwood and Normal Wood of Subalpine Fir in Relation to Drying

Subalpine fir (Abies lasiocarpa Hook) lumber containing wetpockets (wetwood) was used for the measurements of gas permeability and the results were then compared to the gas permeability of normal wood, which was free of the wetpockets. The impacts of pretreatment methods were also investigated, including steaming at green condition, steaming at fiber saturation point (FSP), microwave treatment, and radio-frequency treatment. Results from this study demonstrated that both the transverse and the longitudinal gas permeability of subalpine fir wetwood are greater than that of normal wood. There is no significant change in the permeability after 4-h steaming pretreatment at green condition and at FSP. The impacts of microwave and radio-frequency pretreatments were not significant, but the findings cannot be generalized because they may depend on the power intensity applied.     

The surgical treatment of acute myocardial infarct in the 90s]

At present, the treatment for acute myocardial infarction is revascularization during the critical initial period of six hours after the beginning of coronary occlusion. Despite the fact that surgery performed within this time period presents a hospital of mortality around 2%, and with excellent results in the long term, it is seldom used due to logistic limitations and capabilities of hospital infrastructures, high costs and the possibility of the surgical team initiating surgery inside the useful time period. Surgery is thus limited to the patients with suitable anatomy, who are not candidates or had failure of thrombolytic/angioplasty therapy and are in the six-hour period after initiation of symptoms. Surgery performed at a later stage has good results if performed in a non emergency situation, specially after the first 72 hours. Surgery continues to be the only treatment for the mechanical complications of infarction, and good results have recently been shown in ventricular septal ruptures, with hospital mortality of 14%, due to the use of an endoventricular patch in patients operated early, before the consequences of low cardiac output develop at systemic level. In the surgical treatment of mitral regurgitation, the tendency has been to use repair techniques whenever possible, but still with hospital mortality up to 15%. The recent advances of the techniques and tactics of myocardial preservation during surgery have made a very significant contribution to the better results we see today.     

Down modulation of MHC surface molecules on B cells by suppressive immune complexes obtained from chronic intestinal schistosomiasis patients

The effect of repeated maximal isometric knee extensions on electromechanical delay (EMD) and associated muscle temperature changes were investigated on seven college aged subjects. The exercise produced a significant reduction in muscle contraction force, rate of force development and muscle conduction velocity, whilst the muscle temperature increased by 2.1 degrees C. The EMD increased from a pre-exercise value of 38.4 (SEM 3.4) ms to 55.7 (SEM 3.4) ms post-exercise. In an attempt to evaluate the effect of muscle temperature on EMD, hot and ice-water bags were placed on the quadriceps muscle to alter muscle temperature. The EMD in isometric maximal knee extension was measured at 38, 36, 34, 32 and 30 degrees C. The results showed that the EMD elongated at muscle temperatures either lower or higher than 36 degrees C. It was speculated that the increased muscle temperature might contribute to 20-25% of the EMD elongation found during the fatiguing intermittent exercise. The information of the effects of muscle temperature on EMD could be useful when evaluating the effects of strenuous exercise, in which a substantial muscle temperature change might occur, on the time delay between myoelectrical activity and force generation.     

Inter-observer agreement in analysis of basal body temperature graphs from infertile women

In this study, we evaluated the reproducibility of analysis of basal body temperature graphs under optimized conditions for agreement. A total of 160 recordings were selected from spontaneous cycles of infertile women and analysed by three experienced clinicians using uniform criteria. Agreement at the various stages of analysis was assessed by the 'proportions of agreement' with 95% confidence intervals. Agreement in identification of the thermal nadir was clearly superior to that reported in previous publications. Reproducibility of 'ovulatory' graph features (i.e. biphasic graphs and adequate thermal shifts) was excellent. Agreement in classification of monophasic graphs and inadequate thermal shifts, although lower, was still good. Thus, with experienced observers and uniform criteria, a good agreement can be achieved in analysis of the most important parameters of the basal body temperature graph. We believe that an effort should be made to generalize the use of uniform analysis criteria, because only then can results from different institutions be compared and the remaining clinical evaluation of the method be performed.     

Gain‐scheduled dynamic output feedback control for discrete‐time LPV systems

This paper presents synthesis conditions for the design of gain‐scheduled dynamic output feedback controllers for discrete‐time linear parameter‐varying systems. The state‐space matrix representation of the plant and of the controller can have a homogeneous polynomial dependency of arbitrary degree on the scheduling parameter. As an immediate extension, conditions for the synthesis of a multiobjective ??_∞ and ??₂ gain‐scheduled dynamic feedback controller are also provided. The scheduling parameters vary inside a polytope and are assumed to be a priori unknown, but measured in real‐time. If bounds on the rate of parameter variation are known, they can be taken into account, providing less conservative results. The geometric properties of the uncertainty domain are exploited to derive finite sets of linear matrix inequalities based on the existence of a homogeneous polynomially parameter‐dependent Lyapunov function. An application of the control design to a realistic engineering problem illustrates the benefits of the proposed approach. Copyright © 2011 John Wiley & Sons, Ltd.     

Analysis of weakly guiding cladded acoustic fibers of hexagonal crystal symmetry

An approximate analysis of the weakly guiding cladded acoustic fiber consisting of a core and infinite thick cladding both with hexagonal crystal symmetry is presented. The crystalline Z axis is parallel to the fiber axis. Weak guidance conditions require that the stiffness constants of the core are slightly less than those of the cladding, and the density of the core and cladding are almost the same. Approximate dispersion and cutoff equations are derived for all pure guided modes. Dispersion curves of several lower-order guided torsional, radial-axial and flexural modes are evaluated using both exact and approximate formulas and they are in good agreement. A simple approximate dispersion equation for leaky (longitudinal-type) modes is also obtained.